What is pUC19 used for?

What is pUC19 used for?

pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number.

What does pUC19 mean?

plasmid cloning vectors
pUC19 is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers. The designation “pUC” is derived from the classical “p” prefix (denoting “plasmid”) and the abbreviation for the University of California, where early work on the plasmid series had been conducted.

Does pUC19 have ampicillin resistance?

The pUC19 plasmid confers ampicillin resistance to its host through its bla gene which codes for a β-lactamase that cleaves the β-lactam ring in ampicillin. The pUC19 plasmid also contains the lacZ gene which encodes the N-terminal fragment of the enzyme beta-galactosidase.

What type of plasmid is pUC19?

E. coli plasmid
Description. Thermo Scientific pUC19 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC18 vector except that it is arranged in opposite orientation.

What is the difference between pBR322 and pUC19?

The three proposed explanations lead to a common effect: pUC19 yields a higher plasmid copy than pBR322. When present individually in a cell, pUC19 has a plasmid copy number of around 75 per cell at 37⁰C and >200 per cell at 42⁰C, whereas pBR322 has a plasmid copy number of only around 20 per cell (7, 4).

What is pBR322 vector?

pBR322 DNA is a commonly used plasmid cloning vector in E. coli (1). The molecule is a double-stranded circle 4,361* base pairs in length (2). pBR322 contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol.

What is Ori in plasmid?

The replicon is comprised of the origin of replication (ori) and all of its control elements. The ori is the place where DNA replication begins, enabling a plasmid to reproduce itself as it must to survive within cells.