What is the function of T7 RNA polymerase?
What is the function of T7 RNA polymerase?
What is the function of T7 RNA polymerase?
T7 RNA polymerase is a very active enzyme: it synthesizes RNA at a rate several times that of E. coli RNA polymerase and it terminates transcription less frequently; in fact, its transcription can circumnavigate a plasmid, resulting in RNA several times the plasmid length in size.
What is the molecular weight of T7 RNA polymerase?
98,856
The calculated molecular weight for T7 RNA polymerase is now 98,856.
Where does T7 RNA polymerase originate from?
bacteriophage T7
T7 RNAP is a small (99 kDa) DNA-dependent RNAP derived from bacteriophage T7 (Chamberlin and Ring, 1973; Golomb and Chamberlin, 1974; Steitz, 2004). The polymerase efficiently and specifically transcribes genes bearing a T7 promoter (PT7).
Why is the T7 promoter used?
The T7 promoter is commonly used to regulate gene expression of recombinant proteins, which can be subsequently used for a variety of downstream research applications2.
Is the T7 virus common?
Bacteriophage T7 (or the T7 phage) is a bacteriophage, a virus that infects bacteria. It infects most strains of Escherichia coli and relies on these hosts to propagate….T7 phage.
| Escherichia virus T7 | |
|---|---|
| Species: | Escherichia virus T7 |
Where is T7 found in the world?
The T7 vertebra is the seventh thoracic vertebra, found in the middle of the chest between the seventh and eighth pairs of ribs. It plays important roles in the support of the spinal cord, ribcage, and muscles of the chest.
Does E coli have T7 polymerase?
Protein expression in Escherichia coli (E. The T7 RNA Polymerase System is the most popular approach for producing proteins in E. coli. In this system, an expression vector containing a gene of interest, cloned downstream of the T7 promoter, is introduced into a T7 expression host.
Is T7 a phage?
Bacteriophage T7 (or the T7 phage) is a bacteriophage, a virus that infects bacteria. It infects most strains of Escherichia coli and relies on these hosts to propagate. Bacteriophage T7 has a lytic life cycle, meaning that it destroys the cell it infects.