What does the Lineweaver-Burk plot show?
What does the Lineweaver-Burk plot show?
What does the Lineweaver-Burk plot show?
The Lineweaver–Burk plot was widely used to determine important terms in enzyme kinetics, such as Km and Vmax, before the wide availability of powerful computers and non-linear regression software. The y-intercept of such a graph is equivalent to the inverse of Vmax; the x-intercept of the graph represents −1/Km.
What are the limitations of the Lineweaver-Burk plot?
Figure 6-5a shows a Lineweaver—Burk plot. The disadvantage of this plot is that it depends on less precisely determined points obtained at low values of [S], whereas the more accurate points obtained at high values of [S] cluster and so are less valuable in establishing the linear plot.
What is the Lineweaver-Burk equation?
The Lineweaver-Burk equation is a linear equation, where 1/V is a linear function of 1/[S] instead of V being a rational function of [S]. The Lineweaver-Burk equation can be readily represented graphically to determine the values of Km and Vmax.
Why is Lineweaver-Burk plot more accurate?
For instance; Lineweaver-Burke plot, the most favoured plot by researchers, has two distinct advantages over the Michaelis-Menten plot, in that it gives a more accurate estimate of Vmax and more accurate information about inhibition. It increases the precision by linearizing the data.
Why are Lineweaver-Burk plots inaccurate?
Enzyme kinetic data that lies closest to the origin on a Lineweaver-Burk plot must have the smallest 1/[S] and 1/V values. Thus relying too heavily on the points far from the origin can lead to inaccurate values of KM and Vmax.
What are the advantages of the Lineweaver-Burk plot?
What is the formula for calculating Vmax?
v = Vmax – Km x v / [S]
- y intercept = Vmax.
- gradient = -Km.
- x intercept = Vmax / Km.
Why is Hanes Woolf better than Lineweaver-Burk?
Just like the Lineweaver-Burk equation, the Hanes-Woolf equation is of the form y = mx + c. The Hanes-Woolf plot is thought to be more accurate than Lineweaver-Burk for the determination of kinetic parameters.
What is the importance of Lineweaver-Burk equation?
Uses of Lineweaver–Burk Plot Used to determine important terms in enzyme kinetics, such as Kmand Vmax, before the wide availability of powerful computers and non-linear regression software. Gives a quick, visual impression of the different forms of enzyme inhibition.
Who is the author of the Lineweaver-Burk plot?
In biochemistry, the Lineweaver–Burk plot (or double reciprocal plot) is a graphical representation of the Lineweaver–Burk equation of enzyme kinetics, described by Hans Lineweaver and Dean Burk in 1934.
When to use a reciprocal Lineweaver-Burk plot?
One creates a secondary, reciprocal plot: 1/velocity vs. 1/ [substrate]. When catalytic activity follows Michaelis-Menten kinetics over the range of substrate concentrations tested, the Lineweaver-Burk plot is a straight line with
Which is the independent axis of Lineweaver-Burk plot?
The independent axis of Lineweaver- Burk plot is the reciprocal of substrate concentration. V 0 or V: initial velocity of an enzyme inhibited reaction. The dependent axis of the Lineweaver- Burk plot is the reciprocal of velocity. V max: maximum velocity of the reaction.
How is the Lineweaver Burk plot used in enzyme kinetics?
The Lineweaver–Burk plot was widely used to determine important terms in enzyme kinetics, such as Km and Vmax, before the wide availability of powerful computers and non-linear regression software. The y -intercept of such a graph is equivalent to the inverse of Vmax; the x -intercept of the graph represents −1/ Km.