What is a Boyden chamber assay?

What is a Boyden chamber assay?

The Boyden chamber is a useful tool to study cell migration and cell invasion. It consists of a cylindrical cell culture insert nested inside the well of a cell culture plate. The insert contains a polycarbonate membrane at the bottom with a defined pore size.

What is Boyden chamber experiments?

The most widely accepted cell migration technique is the Boyden Chamber assay. The classic Boyden Chamber system uses a hollow plastic chamber, sealed at one end with a porous membrane. Cells are placed inside the Chamber and allowed to migrate through the pores, to the other side of the membrane.

What are migration assays?

Introduction. The transwell migration assay is a commonly used test to study the migratory response of endothelial cells to angiogenic inducers or inhibitors. This assay is also known as the Boyden or modified Boyden chamber assay.

What is cell migration assay used for?

The transwell cell migration assay measures the chemotactic capability of cells toward a chemo-attractant. The transwell cell invasion assay, however, measures both cell chemotaxis and the invasion of cells through extracellular matrix, a process that is commonly found in cancer metastasis or embryonic development.

What is the migration of leukocytes?

Leukocyte migration involves passage from the tissues to the blood and lymphatic vessels and from the vessels to the tissues (extravasation). Cells undergo a multistep process to bind the vessel endothelium.

What is leukocyte transendothelial migration?

Inflammation is tightly regulated by the body and is associated with transient crossing of leukocytes through the blood vessel wall, a process called transendothelial migration (TEM) or diapedesis. TEM is a close collaboration between leukocytes on one hand and the endothelium on the other.

What is Matrigel invasion assay?

The Invasion Assay provides an in vitro system to study cell invasion of malignant and normal cells. Corning Matrigel matrix serves as a reconstituted basement membrane in vitro, occluding the pores of the membrane and blocking non-invasive cells from migrating through the membrane.

How does migration assay work?