How do you differentiate N2a cells?

How do you differentiate N2a cells?

N2a were differentiated where stated by incubating cells in the DMEM supplemented with 0.5% (vol/vol) FBS and 0.5 mM N6,O2′-Dibutyryl cyclic adenosine 3,5-monophosphate (dbcAMP, Sigma-Aldrich) or in reduced serum medium (DMEM supplemented with 0.5% (vol/vol) FBS without retinoic acid) (2).

What is a N2a cell line?

Neuro 2A (N2a) is a mouse neural crest-derived cell line that has been extensively used to study neuronal differentiation, axonal growth and signaling pathways. A convenient characteristic of these cells is their ability to differentiate into neurons within a few days.

What are SH SY5Y cells?

SH-SY5Y is a thrice-subcloned cell line derived from the SK-N-SH neuroblastoma cell line. It serves as a model for neurodegenerative disorders since the cells can be converted to various types of functional neurons by the addition of specific compounds.

What are N2 cells?

N2 Supplement-A, containing iron-rich human transferrin, was developed for the in vitro differentiation of mouse or human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells to neural and pancreatic-like cell types.

Are SH-SY5Y cells adherent?

The cell line SH-SY5Y are semi adherent cell lines, meaning that they have the property to grow as adherent cultures as well as a suspension culture. I would suggest adding Bovine serum albumin to the flask to improve adhesion. When I checked the cells during the first three days there were very few floating cells.

How do you freeze SH-SY5Y cells?

Freeze early passages of SH-SY5Y neuroblastoma cells in Basic Growth Media supplemented with 5% (v/v) DMSO. Initially, freeze aliquots at -80 °C for 24 hr, then transfer to liquid nitrogen for long-term storage.

What is N2 medium?

NDiff N2 medium is a proprietary, completely defined, serum-free cell-culture supplement for the derivation, maintenance, expansion, and/or differentiation of human and mouse stem cells and tissue-derived neural stem (NS) cells.

What does N2 supplement contain?

human transferrin
N2 Supplement-A, containing iron-rich human transferrin, was developed for the in vitro differentiation of mouse or human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells to neural and pancreatic-like cell types.

Are SH-SY5Y cells neurons?

What are SK-N-SH cells?

SK-N-SH is a neuroblastoma cell line that displays epithelial morphology and grows in adherent culture. Treatment with all-trans-retinoic acid causes these cells to differentiate and adopt a neuronal phenotype, characterized by extensive neurite outgrowth.

What is in N 2 supplement?

A serum-free, chemically defined, concentrated media supplement formulated to provide optimal growth conditions for neural stem cell expansion (Catalog # AR003). N-2 Plus is composed of Bovine Insulin, Human Transferrin, Putrescine, Selenite, and Progesterone. The supplement is supplied as a 100X concentrate in water.