What are RAW264 7 macrophages?

What are RAW264 7 macrophages?

7 cells are monocyte/macrophage like cell linage, originating from Abelson leukemia virus transformed cell linage derived from BALB/c mice. 21 Initially, RAW264. 7 cells have been described as an appropriate model of macrophages, as the cell are capable for performing pinocytosis and phagocytosis.

How do you revive a raw cell?

Thaw frozen cells rapidly (< 1 minute) in a 37°C water bath. Dilute the thawed cells slowly before you incubate them, using pre-warmed growth medium. Plate thawed cells at high density to optimize recovery. Always use proper aseptic technique and work in a laminar flow hood.

What are J774 cells?

Macrophage cell lines like J774 cells are ideal model systems for establishing the biophysical foundations of autonomous deformation and motility of immune cells. Passive J774 cells cultured in suspension exhibited a cortical resting tension of ∼0.14 mN/m and a viscosity (at room temperature) of 0.93 kPa·s.

How are cell lines made?

In order to get embryonic stem cell lines, scientists remove cells from the inner cell mass region. Once the cells are removed, they are placed on a culture plate with nutrients and growth factors. The blastocyst is destroyed in this process. An embryonic cell line is established when these cells multiply and divide.

What is raw cell line?

RAW 264.7 cells are a macrophage-like, Abelson leukemia virus-transformed cell line derived from BALB/c mice. This cell line is a commonly used model of mouse macrophages for the study of cellular responses to microbes and their products. RAW-Blue™ cells express the secreted alkaline phosphatase (SEAP) reporter gene.

How long does it take for RAW cells to adhere?

If you trypsinized the adherent cells then replate them probably only need a few minutes for some of them to attach. For both endothelial and sommoth muscle cells I have seen them attached withing 20 minutes whereas from liquid N2 it may take 2-4 hours.

What are primary macrophages?

Bone marrow-derived macrophages (BMM) are primary macrophage cells, derived from bone marrow cells in vitro in the presence of growth factors.

What are RAW 264.7 cells used for?

The RAW 264.7 cells are monocyte/macrophage-like cells, originating from Abelson leukemia virus transformed cell line derived from BALB/c mice. These cells are being described as an appropriate model of macrophages. They are capable of performing pinocytosis and phagocytosis.