What are recognition sites in biology?

What are recognition sites in biology?

What are recognition sites in biology?

A nucleotide sequence—composed typically of 4, 6, or 8 nucleotides–that is recognized by a Restriction endonuclease.

What is a restriction endonuclease recognition site?

A restriction enzyme, restriction endonuclease, or restrictase is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. These enzymes are found in bacteria and archaea and provide a defense mechanism against invading viruses.

What is the recognition site for EcoRI?

Additional Information: The EcoRI is a restriction enzyme that creates four nucleotide sticky ends with the end of 5′. The enzyme cuts at the recognition site of G/AATTC which has a complementary sequence of CTTAA/G.

What is an endonuclease site?

Endonucleases are enzymes that cleave the phosphodiester bond within a polynucleotide chain. The nucleotide sequence recognized for cleavage by a restriction enzyme is called the restriction site. Typically, a restriction site will be a palindromic sequence about four to six nucleotides long.

What are DNA fragments called?

A restriction fragment is a DNA fragment resulting from the cutting of a DNA strand by a restriction enzyme (restriction endonucleases), a process called restriction. Restriction fragments can be analyzed using techniques such as gel electrophoresis or used in recombinant DNA technology.

What is a sticky end in biology?

After digestion of a DNA with certain Restriction enzymes, the ends left have one strand overhanging the other to form a short (typically 4 nt) single-stranded segment. This overhang will easily re-attach to other ends like it, and are thus known as “Sticky ends”.

What is EcoRI an example of?

EcoRI is an example of a type II restriction enzyme. There is evidence that restriction enzymes of this type are evolutionarily related.