What is pGEX vector?

What is pGEX vector?

pGEX-2TK is designed to allow the detection of expressed proteins by directly labeling the tagged products in vitro. This vector contains the recognition sequence for the catalytic subunit of cAMP-dependent protein kinase obtained from heart muscle.

What is pGEX 2t?

Bacterial vector for expressing GST fusion proteins with a thrombin site.

What is a pET vector?

pET Bacterial Recombinant Protein Vector. Overview. The pET vector system is a powerful and widely used system for expressing recombinant proteins in E. coli. The gene of interest is cloned into the pET vector under the control of the strong bacteriophage T7 transcription and translation regulatory system.

What is a thrombin site?

A thrombin cleavage site (e.g., Leu-Val-Pro-Arg-ll-Gly-Ser; where ll denotes the cleavage site) is widely incorporated within the linker region of fusion or affinity tagged recombinant proteins. The kit contains active thrombin enzyme sufficient to cleave up to 5 mg of the target protein.

Is IPTG a gene?

IPTG (Isopropyl ß-D-1-thiogalactopyranoside), is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon and it is therefore used to induce protein expression where the gene is under the control of the lac operator.

What is the code for the pGEX vector?

Simplified package with only the pGEX vector in the pack ( E. coli BL21 will continue to be available separately, code no 27-1542-01. Updated Product Specification sheets.

Which is the correct reading frame for pgex-4t-2?

Bacterial vector for expressing fusion proteins with a thrombin site. For other reading frames, use pGEX‑4T-1 or pGEX‑4T-2. To see this sequence with restriction sites, features, and translations, please download SnapGene or the free SnapGene Viewer.

Is the Addgene vector database available for free?

This is a free resource for the scientific community that is compiled by Addgene. This page is informational only – this vector is NOT available from Addgene – please contact the manufacturer for further details.

When to use Apai or EcoRV on pgex-2t?

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. BssHII is typically used at 50°C, but is 75% active at 37°C. ApaI can be used between 25°C and 37°C.